Thursday, July 21, 2011

How do plant's alter the earth's atmosphere by removing carbon?

First of all, we must understand the effect of excess carbon in the atmosphere.  Excess carbon includes mostly carbon dioxide as well as water vapor and methane.

Excess carbon in the atmosphere  increases the temperature of the earth by absorbing infrared and near infrared light. (ref:  http://www.ideaconnection.com/solutions/7510-Excess-Carbon-Dioxide-in-Atmosphere.html)

Carbon dioxide is a greenhouse gas.

Greenhouse effect  refers to circumstances where the short wavelengths of visible light from the sun pass through a transparent medium and are absorbed, but the longer wavelengths of the infrared re-radiation from the heated objects are unable to pass through that medium. The trapping of the long wavelength radiation leads to more heating and a higher resultant temperature. Besides the heating of an automobile by sunlight through the windshield and the namesake example of heating the greenhouse by sunlight passing through sealed, transparent windows, the greenhouse effect has been widely used to describe the trapping of excess heat by the rising concentration of carbon dioxide in the atmosphere.

The increasing amount of carbon dioxide in the atmosphere is increasing the temperature of the earth by absorbing infrared and near infrared light.
 
How do plants help remove excess carbon?  Photosynthesis
 
Plants use the energy from the sun to remove carbon dioxide from the atmosphere and use it to make carbohydrates (sugars).
 
Plants take up CO2 (carbon dioxide) from the atmosphere and uses it for the metabolic production of sugars.  Carbon dioxide is fixed or incorporated into specific organic molecules by the Calvin Cycle which is the second stage of photosynthesis.
 
 
 

Understanding the Krebs Cycle

What is the Krebs Cycle?

The Krebs Cycle is the third stage of cellular respiration.  It is a cyclic metabolic pathway in which cells use pyruvate from glycolysis to produce 3NADH and 1FADH2,  2CO2, and 1 ATP per turn.

NADH and FADH2 are electron carriers.  These two molecules are shuttled to the inner mitochondrial membrane to be used by the Electron Transport Chain (more on that later).

The Krebs Cycle occurs in the mitochondrial matrix.

Pyruvate from glycolysis enters the mitochondrion via the pyruvate dehydrogenase complex (PDHC) which is a multienzyme complex located in the outer mitochonrial membrane. 

The PDHC compelx converts pyruvate to acetyl-CoA.

Two pyruvates are formed at the end of glycolysis.  Therefore,

2Pyruvate  ----->  2acetly-CoA   +  2 CO2

Remember, the overall equation for cellular respiration is:

C6H12O6   + 6O2  -------->  6CO2   +   6H20   +  energy (ATP)
glucose     +  oxygen  -----> carbon dioxide  +  water  +  energy

Glycolysis converts the glucose to pyruvate.

Pyruvate is converted to acetyl-CoA yielding two carbon dioxide molecules.  The other 4 CO2 are produced during the Krebs Cycle.

Sunday, June 12, 2011

Second Half of Glycolysis

The last five reactions of glycolysis leads to production of two pyruvate molecules.  The last five reactions are called the energy harvesting or energy payoff phase.

Reaction 6:  Glyceraldehyde-P  Dehydrogenase Reaction
G3P is converted to 1, 3 bisphosphoglycerate

This is the first reaction in glycolysis to generate a NADH molecule.  NADH is an electron carrier that is used in the mitochondrion by the ETC to generate ATP.

Reaction 7:  Phosphoglycerate kinase converts 1,3 BPG to 3-phosphoglycerate.  It is the first step of glycolysis to generate ATP via substrate level phosphorylation.  A phosphate group is removed from 1,3 BPG and transferred to ADP to form ATP (substrate-level phosphorylation).

Reaction 8:  Phosphoglycerate mutase converts 3-phosphoglycerate to 2-phosphoglycerate. 

What is the purpose of this reaction?
This rearrangement of the phosphate from the C-3 to C-2 position allows productio of phosphoenolpyruvate in the next reaction.

Reaction 9:  Enolase converts 2-PG to PEP

Reaction 10:  Pyruvate kinase converts PEP to pyruvate and also generates ATP via substrate level phosphorylation.

Summary of the First Five Reactions in Glycolysis

The first five reactions of glycolysis are referred to as the energy investment phase or energy input phase.  These reactions initially use ATP.  Reaction 1 uses an ATP molecule to convert glucose to glucose 6-phosphate.  This input of energy is similar to priming a pump.  Some energy must be used to initiate or start the reactions of the glycolytic pathway.Reaction 3 uses a second ATP molecule to convert F6P to F 1,6-BP. 

The first phase converts glucose (a 6 carbon molecule) to two glyceralde 3-phosphate molecules (3 carbon molecules).

Steps 4 & 5 of Glycolysis

In step 4 fructose 1,6-bisphosphate is cleaved by fructose bisphosphate aldolase to form dihydroxyacetone phosphate (DHAP) and glyceraldehyde 3-phosphate (G3P).  This reaction is unfavorable written at standard state.  Cellularly, the change in free energy is close to zero. 

In step 5 DHAP is converted to G3P by triose phosphate isomerase.

Why is DHAP converted to G3P?
The purpose of this reaction is to allow both products of the aldolase reaction to continue in glycolysis.

Friday, May 27, 2011

Step 3: The Committed Step in Glycolysis

In step 3 fructose-6-phosphate is converted to fructose 1,6 bisphosphate by the enzyme phosphofructokinase (PFK-1).  Phosphofructokinase is highly regulated inside the cell. 

Why is step 3 the committed step in glycolysis?

Step 3 is an irreversible reaction whereas steps 1 & 2 are reversible, however, equilibrium of these first two reactions lie to the right in favor of the reaction going forward.  The conversion of fructose-6-phosphate to fructose 1,6, bisphosphate commits the cell to carrying out glycolysis.

Overview of Reactions of Steps 1 & 2 of Glycolysis

                                       Hexokinase
Step 1:  Glucose + ATP  ------------>  Glucose-6-Phosphate   +  ADP  +  H+
                                            <--

                                               Phosphoglucose
Step 2:  Glucose-6-Phosphate  ----------------> Fructose-6-Phosphate
                                                   isomerase